Toxicity identification and evaluation of interstitial water sample of submarine sewage outfall of Santos (SP), Brazil

Mariana Aliceda Ferraz, Rodrigo Brasil Choueri, Aline Vechio Alves, Fabio Hermes Puscedu, Fernando Sanzi Cortez, Camilo Dias Seabra Pereira, Augusto Cesar


The methods of Toxicity Identification and Evaluation (TIE) combine the toxicity tests and simple manipulations physical / chemical properties of a sample in order to identify the substances responsible for the observed toxicity. The aim of this study was to identify substances that cause toxicity in the vicinity of the submarine sewage outfall of Santos (SP) (SSO), Brazil. Sediments of Bertioga (SP) were used as reference. We used the following manipulations: (i) the addition of EDTA in the sample, (ii) passing the sample through a C18 reverse phase column, (iii) aeration of the sample acidified (pH 3.0) (v) aeration of the sample with pH modified to 9.0. Results of toxicity of samples prior to manipulations (pre-manipulation) and after the manipulations (post-manipulation) were compared by Dunnett test (α = 0.05). The interstitial water sample Bertioga showed no initial toxicity. The method of AIT showed the toxicity of the interstitial water is caused by acid volatile compounds (probably sulfides).

Key words

Toxicity Identification and Evaluation (TIE); Lytechinus variegatus.; submarine sewage outfall of Santos - SP.

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